Abstract:
Over the past half century, the human population has seen a significant increase in the
proportion of individuals exposed to ionizing radiation. One of the urgent problems of
radiobiology is the establishment of the natural radiosensitivity of the organism, which
provides the possibility of predicting the remote consequences of radiation exposure. The
general condition of animals after ionizing radiation and the changes caused by this radiation
largely determine the functioning of muscle tissue, which plays an important role in ensuring
the vital activity of the body. The leading pathogenetic factor of radiation damage to the body
is a violation of the metabolism and structural organization of enzymatic systems, due to which
a sequence of biochemical processes and compartmentalization of enzyme systems change,
and their solubilization increases. Additionally, it is extremely important to understand the
detailed mechanisms of energy supply for the functioning of muscle tissue in the body of intact
animals. The purpose of the work is to determine the pathophysiological significance of
creatinekinase and lactatedehydrogenase in the mechanisms of adaptation of the body of the
descendants of intact rats to the influence of a stress factor by studying changes in the activity
of creatinekinase and lactatedehydrogenase in the blood and muscle system. A characteristic
feature of creatine metabolism in 1-month-old pup rats was shown to be the low content of
creatine and creatinine in skeletal and cardiac muscles against the background of low activity
of the creatine phosphokinase enzyme in these muscles and its sharp increase in blood.
Expressed hypercreatinuria and hypocreatininuria are registered in the blood of 1-month-old
pup rats, which can be realized due to the strengthening of biosynthetic processes against the
background of the inability of muscle tissue to fix these metabolites. In the skeletal and cardiac
muscles of intact 1-month-old pup rats, the activity of creatine phosphokinase is decreased due
to the decrease in the activity of the MM -isoform of the enzyme. The investigated metabolic
processes in the muscle system of intact animals were shown to be extremely sensitive to the
altering influence of ionizing radiation. The authors stressed that understanding the
pathophysiological mechanisms of adaptation of the muscular system to the probable influence
of a stressful radiation factor, we can draw a conclusion about the depth and severity of the
pathological process based on the state of the enzyme systems, and we consider the
determination of enzymatic tests in the blood to be important in diagnostic and prognostic
aspects.
