Анализ иммунологических и биохимических показателей у больных хроническим гепатитом С в зависимости от генотипа вируса

Abstract

В статье представлены исследования биохимических, генетических и иммунологических показателей у 100 больных хроническим гепатитом С, проживающих в Одесском регионе. Проведен корреляционный анализ между этими показателями и генотипом вируса с целью выявления возможных маркеров тяжести течения заболевания.

The pathogenic features of chronic hepatitis C are determined by interaction of two main factors: human body’s characteristics and the structure of HCV (pathogen). The body’s features include the nature of the immune response. There is an interrelation between HCV genotypes, the nature of the immune response, and progression of liver fibrosis. However, studies on the immunological indices, cytokine profile depending on the genotype are few. The objective: to analyze the immunological and biochemical indices in the patients with chronic hepatitis C (CHC), depending on the viral genotype. Materials and methods. There were examined 100 patients with chronic hepatitis, aged 18 -62 y.o., were examined. All patients were evaluated traditional biochemical indices. In order to confirm the diagnosis the PCR, qualitative and quantitative determination of viral RNA and the genotype of the hepatitis C virus was carried out in all patients. Results. To assess the interrelation between nonparametric (hepatitis C virus genotype) and parametric (biochemical and immunological indices) data, a Spearman rank correlation coefficient was used. The following regularities were revealed: • a weak positive (direct) association between the virus genotype and the percentage of CD3 +, CD4 + and CD16 + (in patients with chronic hepatitis C caused by genotypes 1a and 1b, fewer amount of CD3 +, CD4 + and CD16 + was observed than in patients with chronic hepatitis C caused by 2 and 3 genotypes ); • a weak negative (inverse) interrelation between the virus genotype and the percentage of CD8 + and CD19 + (patients with chronic hepatitis C caused by genotypes 1a and 1b have a higher number of CD8 + and CD19 + than patients with chronic hepatitis C caused by 2 and 3 genotypes); • moderate negative (inverse) interrelation between the virus genotype and the activity of ALT and AST (in the patients with chronic hepatitis C caused by genotypes 1a and 1b, transaminase activity is higher than in the patients with chronic hepatitis C caused by 2 and 3 genotypes). Conclusion. As a result of the pilot studies, it can be assumed that the genotype of virus 1a and 1b causes more pronounced changes in biochemical indices and immunological status indices than the virus of its third genotype. Perhaps the genotype of the virus affects the rate of progression of fibrosis, which will be the subject of our further research.

У статті представлені дослідження біохімічних, генетичних та імунологічних показників у 100 хворих на хронічний гепатит С, які проживають в Одеському регіоні. Проведений кореляційний аналіз між цими показниками та генотипом вірусу з метою виявлення можливих маркерів тяжкості перебігу захворювання.