У статті представлені дослідження біохімічних, генетичних та імунологічних показників у
100 хворих на хронічний гепатит С, які проживають в Одеському регіоні. Проведений
кореляційний аналіз між цими показниками та генотипом вірусу з метою виявлення
можливих маркерів тяжкості перебігу захворювання.
The
pathogenic features of chronic hepatitis C are determined by interaction of two main factors:
human body’s characteristics and the structure of HCV (pathogen). The body’s features include
the nature of the immune response. There is an interrelation between HCV genotypes, the nature of
the immune response, and progression of liver fibrosis. However, studies on the immunological
indices, cytokine profile depending on the genotype are few. The objective: to analyze the
immunological and biochemical indices in the patients with chronic hepatitis C (CHC), depending
on the viral genotype. Materials and methods. There were examined 100 patients with chronic
hepatitis, aged 18 -62 y.o., were examined. All patients were evaluated traditional biochemical
indices. In order to confirm the diagnosis the PCR, qualitative and quantitative determination of
viral RNA and the genotype of the hepatitis C virus was carried out in all patients. Results. To
assess the interrelation between nonparametric (hepatitis C virus genotype) and parametric
(biochemical and immunological indices) data, a Spearman rank correlation coefficient was used.
The following regularities were revealed: • a weak positive (direct) association between the virus
genotype and the percentage of CD3 +, CD4 + and CD16 + (in patients with chronic hepatitis C
caused by genotypes 1a and 1b, fewer amount of CD3 +, CD4 + and CD16 + was observed than
in patients with chronic hepatitis C caused by 2 and 3 genotypes ); • a weak negative (inverse)
interrelation between the virus genotype and the percentage of CD8 + and CD19 + (patients with
chronic hepatitis C caused by genotypes 1a and 1b have a higher number of CD8 + and CD19 +
than patients with chronic hepatitis C caused by 2 and 3 genotypes); • moderate negative (inverse)
interrelation between the virus genotype and the activity of ALT and AST (in the patients with
chronic hepatitis C caused by genotypes 1a and 1b, transaminase activity is higher than in the
patients with chronic hepatitis C caused by 2 and 3 genotypes). Conclusion. As a result of the pilot
studies, it can be assumed that the genotype of virus 1a and 1b causes more pronounced changes in
biochemical indices and immunological status indices than the virus of its third genotype. Perhaps
the genotype of the virus affects the rate of progression of fibrosis, which will be the subject of our
further research.
В статье представлены исследования
биохимических, генетических и иммунологических показателей у 100 больных
хроническим гепатитом С, проживающих в Одесском регионе. Проведен корреляционный анализ между этими показателями и генотипом вируса с
целью выявления возможных маркеров тяжести течения заболевания.
