Features of the mineralizing efficacy of water-soluble antistressants in bone tissue of male and female rats after stress

Abstract

Stress ends with the development of dysmetabolic syndrome, which leads to disruption of mineralization processes in bone tissue. Aim. To investigate the effect of anti-stress drugs on the mineralizing activity of bone tissue in rats after stress, taking into account the sexual characteristics of the mineralizing index. Methods. Water-soluble preparations of vitamins P were chosen as anti-stressants: “EkSoVit” (soy extract containing isoflavones and protease inhibitors), quercetin (dietary supplement “Anti-stressant – Vitamins P (quercetin)” and for comparison – a preparation of ascorbic acid (vitamin C). Stress was induced in rats by keeping them at −20 °C for 30 minutes. Antistressants in the form of oral gels were administered 3 days before stress and 3 days after stress. The condition of the tissue (femur) was examined on the 4th day after stress. The activity of alkaline phosphatase (ALP), acid phosphatase (AP) was determined, and the mineralization index (MI) and mineralization efficiency (ME) were calculated from the ALP/AP ratio. Results. Mineralizing activity (MA) was the highest in the preparation "Quercetin", and the lowest − in ascorbic acid. Mineralizing efficiency was the highest in the preparation "Quercetin", and the lowest − in ascorbic acid. Indicators of mineralizing efficiency were higher in males. Conclusion. Stress reduces the mineralization index in bone tissue. Antistressants stimulate mineralization processes by increasing the activity of alkaline phosphatase and reducing the level of acid phosphatase. In terms of mineralization efficiency, the most active drug was “Antistressant – Vitamins P (quercetin)”, and the efficiency of all three antistressants was 5-9 times higher in males.