Abstract:
The role of the gut microbiome and environment in influencing mental health and longevity has
been recently determined and highlighted. To identify gut (oral) microbiome signatures and biochemical
markers associated with mental health and longevity in working-age and elderly individuals. To enable the
development of personalized diagnostics and prevention strategies based on mathematical models for
mental-age-relevant diseases. Fecal and blood samples were collected from participants across two cohort
groups, including working-age, elderly local and refugees. Gut microbiota composition was analyzed via
16S rRNA gene sequencing. Blood samples underwent standard biochemical testing, including lipid
profiles, liver and kidney function, glucose levels, and oxidative stress markers. Mental health status was
assessed through validated psychological questionnaires and cognitive screening tools. Correlations between
microbiome patterns, blood biomarkers, and mental health indicators were analyzed using multivariate
statistical methods. PCA were applied to build prognostic models. Participants with greater microbial
diversity and higher abundance of beneficial taxa such as Lactobacillus and Bifidobacterium showed more
favorable biochemical profiles and better mental health scores. Distinct microbiome patterns were observed
between working and elderly individuals, some of which were linked to markers of healthy aging. The
integration of sequencing and clinical data supports the potential of gut microbiota as a predictive tool for
assessing psychological resilience and longevity. Lacticaseibacillus rhamnosus S25 strain was selected as
the potential component of pharmabiotic DefendeX™ for PTSD prevention and treatment. Individual
nutrition plans are created by using the algorithm of Ediens (BioQuantum).
