Abstract:
Aim: is the investigation the effect of different bioflavonoids combination on the indicators of blood
coagulation in animals with diabetic nephropathy (DN).
Methods: the research was carried out on white nonlinear Wistar line rats, weight 240-280 g. A
streptozotocin model was used to reproduce type 2 diabetes. For this purpose, rats were administered
a single intravenous streptozotocin (“SigmaAldrich Chemie GmbH”, Germany) in dose of 65 mg/kg. The
streptozotocin administration was preceded by a high-fat diet.
Results: presents the results of a study of various bioflavonoids on the course of diabetic
nephropathy against the background of type 2 diabetes. It was found that the most pronounced effect
was shown by the use of the combination L-arginine with quercetin. The use of this combination led to
an improvement in the indicators of coagulation hemostasis. Also using L-carnitine and quercetin led to
the decreasing of von Willebrand factor on 68 % compared with the control pathology group which
may indicate about endothelium protective action. The activity of this combination of flavonoids
(quercetin) and L-arginine is related to their pharmacological properties. Quercetin has powerful
antioxidant properties, while L-arginine, a substrate for NO synthase, is a precursor for NO synthesis in
the vascular endothelium, and its use can increase the bioavailability of NO and dilate coronary arteries.
Conclusion: 1. Bioflavonoids effect on the coagulation link of hemostasis by regulating the balance
of indicators that characterize the stages of blood coagulation. The most pronounced effect on the
indicators of the coagulation system in DN was shown by the use of L-arginine in complex with
quercetin. 2. The most pronounced endothelium protective effect was observed when using L-arginine
in complex with quercetin, this indicator decreased on 68 % compared with the control pathology
group. 3. The obtained results indicate that the complex of L-arginine with quercetin is promising in the
complex treatment of diabetic nephropathy.
