Features of Bio Energetic Metabolism in Children Born after Assisted Reproductive Technologies

Abstract

The features of the functioning of key enzymes of bio energetic metabolism in children born with the application of ART at different age periods. A cytochemical analysis of the activity of key mitochondrial enzymes was performed: succinate dehydrogenase (SDH), α-glycerophosphate dehydrogenase and α-GPDH. The material for the analysis was the buccal epithelium. The material was collected by scraping the epithelium from the inner surface of the cheek. The examined children were representatively distributed into age groups: 1st group - children of the first year old (38 children of the main and 20 children of the control group), 2nd group - from 1 to 3 years old (52 children of the main group and 20 children of the control group), 3rd group - from 4 to 7 years old (46 children of the main group and 20 children of the control group). The average age was 2.7 ± 1.9 years. Conclusion: The study of the activity of mitochondrial enzymes SDH and α-GPDH revealed the most pronounced deviations in the functioning of SDH in the group of children from 1 to 3 years old, which was associated with delayed physical development, allergic diseases, prematurity and diseases of the cardiovascular system. A decrease in the concentration of the enzyme GPDH was most expressed in the older group of children from 4 to 7 years old, and was associated with delayed physical development, Broncho-pulmonary diseases, prematurity, and diseases of the digestive system.