The study of antimicrobial properties of film materials based on biopolymers and antiseptics

Abstract

The development of new biomaterials with improved properties is becoming increasingly important in a wide range of applications. However, some of the most sought-after properties are anti-microbial properties, which can help prevent unwanted wound infections, especially in the face of growing antibiotic resistance of bacteria. The aim of the study was to study the effect of antimicrobial biomaterials based on calcium alginate, as a polymer system of local prolonged delivery of quaternary ammonium compounds, on reference and clinical strains of microorganisms. Samples of antimicrobial biomaterials contained decamethoxin (0.03-0.07 wt%), and polymers (polyvinyl alcohol and calcium alginate). Reference and clinical strains of Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa were used for the study. The sensitivity of strains of microorganisms was determined by the disk-diffusion method according to the generally accepted method. The result of antimicrobial activity was assessed after 24 hours. The mean (M), the mean error of the mean (± m), and the criterion for the significance of differences (p) were calculated. The presence of differences between the research data was assessed by the Student's t-criterion. The results were considered reliable at p<0.05. High antimicrobial properties of the studied samples of antimicrobial biomaterials based on calcium alginate and decamethoxin have been established. It was revealed that the samples of polymeric biomaterials have a higher activity against gram-positive microorganisms compared to gram-negative strains. The composition is not inferior to the antimicrobial effect of a solution of decamethoxin and chlorhexidine in relation to all strains of microorganisms.