Одним із найбільш важких захворювань органів черевної порожнини є жовчний перитоніт.
Системна запальна реакція відіграє одну з ключових ролей, як пусковий механізм розвитку перитоніту,
чим обумовлена актуальність дослідження лейкоцитарної ланки при аналізі ефективності нових способів
корекції даної патології. Дослідження виконано на 180 щурах лінії Вістар вагою 180–200 г. Тварини були
розподілені на 4 групи. При аналізі кількості лейкоцитів крові щурів відмічається більш виражена корекція
показників в 4-й групі в порівнянні з третьою на 1-му та 3-му добу експерименту. На 7-му добу виявлено зниження рівню лейкоцитів нижче норми. При дослідженні лейкоцитарного індекса інтоксикації на першу
та третю добу експерименту відмічається його підвищення на фоні експериментального жовчного перитоніту. Результати 4-ї групи свідчать про зниження даного показника на фоні запропонованого способу
санації і більше наближаються до значень норми в порівнянні з 3-ю групою. На 7-му добу експерименту
були отримані дані про зниження лейкоцитарного індексу інтоксикації в 3-й та 4-й групі нижче норми.
Одним из наиболее тяжелых заболеваний органов брюшной полости является желчный перитонит. Системная воспалительная реакция играет
одну из ключевых ролей, как пусковой механизм
развития перитонита, чем обусловлена актуальность исследования лейкоцитарного звена при
анализе эффективности новых способов коррекции данной патологии.
Исследование выполнено на 180 крысах линии
Вистар массой 180–200 грамм. Животные были
разделены на 4 группы. При анализе уровня лейкоцитов крови крыс отмечается более выраженная
коррекция показателей в 4-й группе по сравнению
с третьей, на 1-е и 3-е сутки эксперимента. На
7 сутки эксперимента выявлено снижение уровня
лейкоцитов ниже нормы. При исследовании ЛИИ
на первые и третьи сутки отмечается его повышение на фоне экспериментального ЖП. Результаты
4-й группы свидетельствуют о снижении данного
показателя на фоне предложенного санирования и
больше приближаются к нормальным показателям
по сравнению с 3-й группой. На 7-е сутки эксперимента были полученны данные о снижении лейкоцитарого индекса интоксикации в 3-й и 4-й группе
ниже нормы.
One of the most serious diseases of the abdominal cavity organs is biliary peritonitis.
The aim of the research is to study the leukocyte index of intoxication and the number of leukocytes in experimental gallstone in animals that underwent complex abdominal sanitation.
Materials and methods. The study was performed on 180 rats of the Wistar line weighing 180–200 grams.
Animals were divided into 4 groups: Group 1 – group of intact rats (20 animals). Group 2 – control group – rats,
who modeled biliary peritonitis without further correction (80 animals). Group 3 – rats whose simulated biliary
peritonitis was corrected by abrasion of the abdominal cavity with a solution of furacilin (1: 5000) with further use
of standard antibiotic therapy. Group 4 – rats whose simulated biliary peritonitis was corrected by double sanitation. The first sanitation is detoxification with 0.04% sodium hypochloride solution 12 hours after the second injection of bile. The second sanitation is a mixture consisting of decametoxin compound (10 mg / 50 ml solution),
sodium hyaluronate (250 mg / 50 ml solution), and succinate buffer 6 hours after the first sanitation.
Biliary peritonitis was modeled as follows: a sterile 10% solution of calcium chloride (1 mg / 100 g of body
weight) was administered intramuscularly. There was a focus of aseptic inflammation. After 72 hours, intraperitoneal bile was injected twice with 0.33 ml / 100 g of body weight at intervals of 12 hours. Blood sampling from
the tail vein was performed at the end of the 1st, 3rd and 7th day of the BP model simulation.
Results and discussion:
1. Analysis of leukocyte dynamics. On the 1st day, very significant differences (at a significance level of
p <0.001) between the results of all the study groups were revealed. The second group is characterized by the
maximum increase of the number of leukocytes. The difference in the analysis of the index of the 4th group was
revealed not only in comparison with the 1st and 2nd groups, but also in comparison with the 3rd group, which indicates the greater effectiveness of the method of abdominal sanation proposed by us already for 1 day. Differences
between the results of the 4th and 3rd groups are 92.6% (the results of the 4th group are closer to the norm). On
day 3, a similar pattern was observed in the analysis of each group of animals. The difference between the results
of the 4th and 3rd groups was 81.3%. On the 7th day of the experiment, a decrease in the number of leukocytes was
found below the normal level (on the 1st and 3rd days, their increase was noted). The differences between groups
3 and 1 were at the level of significance p <0.01, and between 4 and 1 – at the level of p <0.001.
2. During the investigation of the LII, the following data were obtained:
On the first and third day of the study, the differences between the 1st, 2nd, 3rd and 4th groups are very significant (p <0.001). The maximum increase was found in the group with experimental HP without correction. At the
same time, the results of the 4th group testify to a decrease in LII on the background of the proposed sanitation, and
are closer to the results of intact animals compared to group 3 (by 80.8% for the first day, and 69.6% for the 3rd). At
the 7th day, LII decreased in the 3rd and 4th groups as compared with the values of the indicator in intact animals).
In this case, differences were found only between 4 and 1 groups, but already at the significance level p <0.05.
Conclusion: During the analyzing the level of leukocytes in the blood of rats, there is a more pronounced
correction of the indices in the 4th group as compared to the third, on the 1st and 3rd days of the experiment. On
the 7th day of the experiment there was a decrease in the level of leukocytes below the norm.
While researching LII for the first and third days, its increase is noted against the background of the experimental FP. The results of the 4th group show a decrease in this indicator against the background of the proposed sanitation and are closer to normal indicators in comparison with the 3rd group.
On the 7th day of the experiment, data were obtained on the decrease in the leukocytosis index of intoxication in groups 3 and 4 below the norm.
