Стимульована ліпополісахаридом активність лімфоцитів крові за умов застосування кетогенної дієти у кіндлінгових щурів

Abstract

Через 4 нед. с момента воспроизведения коразолового киндлинга у крыс наблюдается увеличение выраженности реакции бластной трансформации, вызванной бактериальным лиопополисахардом. Содержание киндлинговых крыс на кетогенной диете (80 % липидов, а также 3,3 % углеводов и 16,7 % белков) вызвало уменьшение выраженности стимулированной бактериальным липополисахаридом пролиферативной активности лимфоцитов.

Incubation of lymphocytes with LPS during 1,5 h was followed by the increase of blast cells, and their number in control was (59.5±4.7)%, while in kindled rats — 83.7%. IAL was 1.47 and 5.13 correspondently (P<0.05). The number of blast cells in rats, which have been given KD during two weeks was 45.3%, while in kindled rats it was 62.4%. IAL was equal to 0.76 and 1.66 correspondently (P<0.05). Those indices were significantly less when compared with groups of kindled rats, which were not given KD (P<0.05). Maintenance of rats on KD during four weeks was followed the net decrease of blast cells in control and experimental groups up to 41.2 and 48.5%. IAL was also reduced up to 0.7 and 0.95 (P>0.05). The heightening of LPS-induced blast transformation of lymphocytes is observed in postponed period of kindling and this effect is blocked by KD.