Хімічна ідентифікація 2-аміно-4,6-дигідроксипіримідинію гексафторосилікату як потенційного карієспрофілактичного агента

Abstract

Здійснено дослідження можливості використання методів хімічного аналізу для ідентифікації 2-аміно-4,6-дигідроксипіримідинію гексафторосилікату, якій був синтезований та охарактеризований раніше як потенційний карієспрофілактичний агент. В експериментах використовували 1%-ві водний та етанольний (96%) розчини солі, процедури аналізу включали ідентифікацію третинного атому нітрогену, гідроксильних груп фенольного типу, ароматичної аміногрупи і гексафторосилікатного аніону. Підтверджено можливість використання реакції утворення іонних асоціатів з азобарвниками тропеолінами для ідентифікації гетероциклічного катіона.

Currently, fluoride preparations play a key role in the treatment and prevention of caries. In recent years, ammonium hexafluorosilicates (AHFS) have been studied as new potential fluorine-containing anti-caries agents, which demonstrate certain advantages in comparison with known fluoride preparations. Earlier, we showed on the example of pyridinium hexafluorosilicates that for the identification of AHFS, along with physicochemical methods of analysis, classical chemical analysis methods can be used, which are distinguished by the simplicity of the corresponding experimental techniques and the availability of starting reagents. The purpose of this work is to demonstrate the capabilities of chemical analysis methods for the identification of 2-amino-4,6-dihydroxypyrimidinium hexafluorosilicate (I), which was synthesized and characterized earlier. Experiments on the chemical analysis of compound I included four stages: identification of the tertiary nitrogen atom, identification of phenolic hydroxyl groups, identification of the aromatic amino group, identification of the hexafluorosilicate anion. Due to the low solubility of I in water, dissolution was carried out at boiling, identification was carried out after cooling the solution. Analysis procedures were also carried out using an ethanolic solution of the salt I. To identify the tertiary nitrogen atom, reactions with general alkaloid precipitating reagents were used, as well as a specific reaction of formation of ionic associates with azo dyes tropeolins 0, 00, 000-II, which are extracted with chloroform. Identification of phenolic hydroxyls in positions 4, 6 was carried out under the action of a silver nitrate solution, and for the analysis of the aromatic amino group in position 2, reactions of formation of azo dyes with resorcinol, aniline and condensation reactions with the participation of salicylaldehyde, p-dimethylaminobenzaldehyde with the formation of the corresponding azomethine dyes were used. Identification of the SiF₆²⁻ anion was carried out by known reactions on the fluoride ion, taking into account its hydrolysis in dilute aqueous solutions with the formation of fluoride ions.