Relationshipof clinical course, biochemical and immunological changes in patients with chronic hepatitis B+C

Abstract

Recent studies have shown that parenteral viral hepatitis is one of the main causes of chronic diffuse liver diseases. The number of patients not only with chronic hepatitis C and B, but also patients with hepatitis of mixed etiology is increasing annually. At the same time, there is a potentiating effect of the association of HBV and HCV with a high risk of developing progressive forms of chronic viral hepatitis or hepatocellular carcinoma. Correlation of the clinical course, biochemical indices in patients with viral mixed infection of the liver have not been studied enough. In the studies of a number of authors, it has been shown that with mixed infection, erased and latent forms of the disease are rarely observed. The aim of the study is to study the immune status and biochemical parameters in patients with chronic hepatitis B+C for a more accurate assessment of the severity of the pathological process. Materials and methods. 62 patients with chronic hepatitis B+C were examined. All the patients under examination were monitored in the hepatological center of the Odessa Municipal Infectious Hospital (Ukraine). The assessment of the severity of the patients' condition was carried out using biochemical indicators. Confirmation of the diagnosis based on determination (qualitatively and quantitatively) HCV RNA and HBV DNA by polymerase chain reaction. Subpopulations of B- and T-lymphocytes (CD19+, CD16+, CD8+, CD4+, CD3+) were determined by the immunofluorescence method using a set of polyclonal and monoclonal antibodies to establish differential antigens of human lymphocytes using the Eurostar immunofluorescence microscope. Results and discussions. When analyzing the main clinical syndromes in dynamics, it was found that in patients with chronic hepatitis B+C with initial of fibrosis (F0-F1), less pronounced changes in the general condition were observed than in patients with moderate (F2) and advanced fibrosis (F3). The presence of a correlation between the degree of fibrosis and immunological indices in patients with chronic hepatitis B+C allows us to use the information obtained as one of the additional criteria for the severity of the pathological process. The expressiveness of changes of immune status indicators can serve as an additional criterion for the degree of morphological abnormalities in liver tissue.