Abstract:
There are many studies on changes in cervix,
however, there are no data about property of cervical tissue to keep
potential for regeneration under influence of immunodeficiency,
which is very important since it allows escaping severe
irreversible changes in tissue. CD34 stem cells are one of
significant indicator for property of tissual regeneration. So, the
purpose of our study was to identify amount of CD34 stem cells
in the cervix under influence of immunodeficiency of infectious
and non-infectious origin.
Materials and methods. Sectional material of reproductive
women was studied. All subjects were divided into 3 groups:
women who were diagnosed with HIV infection; women
who have identified anamnestic and postmortem signs of
alcohol abuse; group of comparison. After routine testing and
immunohistochemical (IHC) staining to CD34, morphometric
measure was performed. We evaluate presence activities of
stromal cells for detection of connection and relationship
between expression of CD34 and the thickness of the cervical
epithelium, relative volumes of condyloma, cervical dysplasia
severity, degree of infiltration of the mucosal lamina propria
by immunocompetent cells. The obtained digital data were
statistically processed.
Results. Morphological investigation revealed changes of
thickness of the cervical stratified squamous nonkeratinized
epithelium up to 714.23±59.21 x 10-6 m in group of HIVinfected women. Relative volumes of condylomas were increased in both investigated groups with presence of pointed,
flat and inverted types. Both low- and high-grade squamous
intraepithelial lesions were detected more often in investigated
goups. Assessment of the degree of infiltration of the mucosal
lamina propria by immunocompetent cells was changed
unevenly with reducing in HIV-group and increasing in alcohol
group. Results of ICH reaction CD34 realized in cytoplasmic
staining with membranous accentuation in all cases of control
group with strong, but reduced level in investigated groups.
Most close connection was observed for cervical dysplasia
severity and CD34 expression.
Conclusions.HIV infection and alcohol abuse have pronounced
pathological effects with cervical changes. The expression of
CD34 is present in 96% of women with immunodeficiency
mainly with strong reaction. It is statistically likely that it does
not depend on such morphological indicators as thickness of the
cervical epithelium, relative volumes of condyloma, degree of
infiltration of the mucosal lamina propria by immunocompetent
cells. The expression of CD34 has statistically close negative
connection with cervical dysplasia severity (r=-0,81) and can be
used for detection of early potential of tissual transformation in
women with immunodeficiency.
