https://repo.odmu.edu.ua:443/xmlui/handle/123456789/6210 2026-04-11T21:47:43Z 2026-04-11T21:47:43Z Mathyshenko, P. M. Appelhans, O. L. Vinner, N. V. Chebotarova, S. O. Матюшенко, П. М. Аппельханс, О. Л. Віннер, Н. В. Чеботарьова, С. О. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/18668 2025-11-07T08:55:35Z 2025-01-01T00:00:00Z

Modulation of monoaminergic neurotransmission restores mnestic impairments in rats with chronic unpredictable stress Mathyshenko, P. M.; Appelhans, O. L.; Vinner, N. V.; Chebotarova, S. O.; Матюшенко, П. М.; Аппельханс, О. Л.; Віннер, Н. В.; Чеботарьова, С. О. The purpose of the study was to investigate the expression of learning and memory processes in rats in chronic unpredictable stress dynamics in conditions of serotoninergic, dopaminergic and adrenergic neurotransmitter systems activity modulation. Experimental studies were performed on the model of chronic unpredictable stress on Wistar rats with previously induced the serotoninergic, dopaminergic and noradrenergic neurotransmitter systems activity activation and suppression. Animals’ ability to learn and their short- and long-term memory expression were determined in the dynamics of used model, The data obtained confirm the expressed mnestic disorders development in rats during the chronic unpredictable stress manifested by deterioration of learning, memory engrams formation and preservation. Mnestic disorders were shown to be developed from the 1st day of chronic unpredictable stress induction, progressed in its dynamics and were maximally expressed from the 14th day of the trial. Serotonin, dopamine and adrenergic neurotransmission suppression starting from the 7th day of the trial significantly impaired rats learning and short- and long-term memory and their activation on 21st day of the trial resulted in mnestic dysfunctions normalization. The authors stressed on monoaminergic neurotransmission functional activity accounting while analyzing the persons cognitive capabilities in chronic stress conditions. The data obtained are experimental evidence of monoaminergic neurotransmission activation clinical effects testing reasonability in chronic stress conditions while performing the treatment regimens for mnestic disorders pathogenetic therapy in patients with chronic unpredictable stress.; Метою дослідження було вивчення вираженості процесів навчання та пам’яті у щурів в динаміці відтворення хронічного непередбачуваного стресу за умов модуляції активності серотонінергічної, дофамінергічної та адренергічної нейромедіаторних систем. Експериментальні дослідження проводили на моделі непередбачуваного хронічного стресу на щурах лінії Вістар, в яких завчасно сприяли активації та пригніченню активності серотонінергічної, дофамінергічної та норадренергічної нейромедіаторних систем. В динаміці означених модельних умов у щурів визначали здатність до навчання, а також вираженість коротко- та довгочасної пам’яті. Отримані дані свідчать про розвиток в динаміці хронічного непередбачуваного стресу мнестичних розладів у вигляді погіршення процесів навчання, а також формування та збереження енграми пам’яті. Мнестичні порушення формуються з 1-ї доби відтворення хронічного непередбачуваного стресу, прогресують в його динамівці та є максимально вираженими, починаючи з 14-ї доби досліду. Пригнічення активності серотонін-, дофамін- та адренергічної нейротрансмісії, починаючи з 7 доби досліду, суттєво погіршувало здатність щурів до навчання та функціонування коротко- та довготривалої пам’яті, а її активація на 21-й добі сприяли відновленню мнестичних функцій. Автори наголошують на важливості урахування функціональної активності моноамінергічної нейротрансмісії при аналізі когнітивних можливостей організму за умов хронічного стресового впливу. Отримані дані є експериментальним доказом доцільності тестування клінічних ефектів активації моноамінергічної нейротрансмісії за умов хронічного стресового впливу при складанні схем патогенетичнї терапії мнестичних розладів у хворих з хронічним стресом.

2025-01-01T00:00:00Z Appelhans, O. L. Pakhomova, O. O. Protunkevych, O. O. Konovalov, M. F. Solomatin, O. B. Аппельханс, О. Л. Пахомова, О. О. Протункевич, О. О. Коновалов, М. Ф. Соломатін, О. Б. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/18292 2025-10-06T08:05:43Z 2025-01-01T00:00:00Z

Disorders of lipid metabolism and activity of enzymes of krebs cycle and pentose phosphate pathway in the modelling of metabolic acidosis and alkalosis in rats Appelhans, O. L.; Pakhomova, O. O.; Protunkevych, O. O.; Konovalov, M. F.; Solomatin, O. B.; Аппельханс, О. Л.; Пахомова, О. О.; Протункевич, О. О.; Коновалов, М. Ф.; Соломатін, О. Б. The aim was to investigate changes in the orientation of cholesterol metabolism and the spectrum of lipids, as well as the activity of key enzymes of the Krebs cycle and the pentose phosphate pathway, regulators of the NADPH/NADP+ ratio, in tissues of rats with disturbances of acid-base state – metabolic acidosis and alkalosis modelled with the aim of unbalanced nutrition: high-protein and carbohydrate (sucrose) diets. Materials and methods. Metabolic acidosis and alkalosis were modelled in rats sticking to diets with excessive ammonium chloride and sucrose. Acid-base balance and lipoprotein cholesterol content were studied in rat blood using conventional methods, then the animals were decapitated, and lipid content and enzyme activity (NADP-dependent IDH and MDH, G6PD and lipase) were determined in liver tissue, cardiac and thigh muscles using TLC. Results and discussion. The development of metabolic acidosis and alkalosis under the influence of diets with excessive ammonium chloride and sucrose was noted. The diet with ammonium chloride promoted lipolysis, activation of lipase, accumulation of under oxidised FFA and cholesterol, decreased the activity of NADP-dependent ICH in all tissues observed and the activity of NADP-dependent MDH in the tissues of the thigh and cardiac muscles of rats, and increased the activity of G6PD in the liver. The sucrose diet increased lipogenesis, cholesterol biosynthesis, and accumulation of FFA in the tissues of the heart and liver, and activated NADP-dependent MDH and IDH in all tissues observed of rats.; Мета – дослідити ліпідний обмін, а також активність Г6ФД, НАДФ-залежних ІЦДГ та МДГ у тканинах щурів у разі моделювання метаболічного ацидозу та алкалозу за допомогою дієт з хлористим амонієм та сахарозою. обидві дієти підвищують вміст холестерину і порушують обмін ліпідів. Встановлено стан метаболічного ацидозу при впливі дієти з хлористим амонієм, активацію ліполізу та накопичення ВЖК, зниження активності НАДФ-ІЦДГ у всіх досліджених тканинах і НАДФ-МДГ у стегновому та серцевому м’язах та активацію Г6ФД у печінки. У разі розвитку метаболічного алкалозу під впливом сахарозного раціону активується ліпогенез та біосинтез ВЖК у тканинах серця та печінки та НАДФ-МДГ i НАДФ-ІЦДГ у всіх тканинах щурів.

2025-01-01T00:00:00Z Mathyshenko, P. M. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/17733 2025-06-12T12:13:25Z 2024-01-01T00:00:00Z

Catecholaminergic neurotransmission activity modulation determines emotional changes in rats with chronic unpredictable stress Mathyshenko, P. M. The emotional component of the stressful nature of our lives is constant, varied in expression and in the nature of emotions. The emotional background often becomes the leading factor determining the severity of stress. We were interested in the peculiarities of catecholaminergic neurotransmitter systems functioning whose activity modulation in conditions of chronic unpredictable stress can significantly change the nature of animals’ emotional reactions. Aim. To study the influence of serotonergic, dopaminergic and adrenergic neurotransmitter systems functional activity modulation on the emotional behavior expression in rats in chronic unpredictable stress.

2024-01-01T00:00:00Z Artemov, О. V. Lytvynenko, М. V. Chumachenko, I. V. Bondarenko, A. V. Dotsenko, N. V. Ostapchuk, K. V. Koshelnyk, O. L. Gargin, V. V. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/16524 2025-01-06T09:48:10Z 2024-01-01T00:00:00Z

The influence of the demodex mite on the morphological picture of eyelid papilloma Artemov, О. V.; Lytvynenko, М. V.; Chumachenko, I. V.; Bondarenko, A. V.; Dotsenko, N. V.; Ostapchuk, K. V.; Koshelnyk, O. L.; Gargin, V. V. Aim: The aim of work is description of new observations related to the participation of demodex in tumor morphogenesis with goal to study the example of eyelid papilloma, imagine the connection between the presence of the demodex mite and certain changes in the typical histomorphological picture of the neoplasm.

2024-01-01T00:00:00Z Artemov, О. V. Lytvynenko, М. V. Neskoromna, N. V. Chebotarova, S. О. Prus, R. V. Oluwafemi, A.T. Nassar, M. Narbutova, Т. Ye. Larson, L. М. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/15637 2024-07-09T07:41:53Z 2024-01-01T00:00:00Z

Pathomorphosis of eyelid tumor pathology in demodectic infection Artemov, О. V.; Lytvynenko, М. V.; Neskoromna, N. V.; Chebotarova, S. О.; Prus, R. V.; Oluwafemi, A.T.; Nassar, M.; Narbutova, Т. Ye.; Larson, L. М. Pathological changes in the tissues of the oculo-conjunctival region caused by the activity of the Demodex mite is represented by the development of inflammatory process. On detection of the parasite on eyelashes, the presence of the Demodex mite is diagnosed in half of adult patients seeking ophthalmic care. Pathomorphological descriptions of specific patterns associated with the presence of Demodex infection are practically absent. During pathological examination, in the vast majority of cases it is almost impossible to see the parasite in the test material. There are pathomorphological patterns associated with the presence of the mite as a commensal, not only on the eyelid surface or in the conjunctiva, but also in morphological structures formed against the background of pathological processes in this area. Aim. To find out the morphological patterns reflecting the pathomorphosis of some tumor processes in the eyelid thickness caused by demodectic invasion, which have not been identified so far in ophthalmopathological studies. Materials and Methods. We analyzed the archival material from the oculo-conjunctival region submitted to the ocular pathology laboratory within the period of 2020–2023. Surgical and biopsy specimens were processed by standard histologic methods. Results. One of the pathognomonic patterns of demodecosis are calcifications with fragments of the dead mite and cysts. When a mite rapidly destroys tissue, it leaves the site until it dies without retaining its fragments. Post-demodecosis pathomorphosis in basal cell carcinoma of the eyelids complicates the pathomorphologic diagnosis of the biopsy. Conclusions. Our results prove the presence of mites in tumor tissues and illustrate their influence on the development of the pathomorphological picture, which should be taken into account in the practical activity of a pathologist.

2024-01-01T00:00:00Z Tregub, T. Lytvynenko, M. Kukushkin, V. Chebotarova, S. Oliynyk, N. Gulbs, O. Nazaryan, R. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/15487 2024-06-11T10:59:04Z 2023-01-01T00:00:00Z

Pharmacology of post-traumatic stress disorder Tregub, T.; Lytvynenko, M.; Kukushkin, V.; Chebotarova, S.; Oliynyk, N.; Gulbs, O.; Nazaryan, R. The aim of this study was to evaluate current approaches to the pharmacotherapy of posttraumatic stress disorder. An information search was carried out in the databases PubMed, Ovid, EMBASE by keywords: “posttraumatic stress disorder”, “treatment”, and “medications”. Search depth 2012–2022 years. From the general data (4877 articles) there were selected 14 articles with the highest degree of relevance. A content analysis of selected articles was carried out with the formation of recommendations for the use of pharmacotherapy in posttraumatic stress disorder. Currently, there are no unified approaches to the pharmacotherapy of posttraumatic stress disorder. Antidepressants (SSRI SNRIs) are primarily considered as first-line drugs, but only sertraline, paroxetine, and fluoxetine are approved by the FDA. But these drugs have a fairly wide range of side effects, including suicidal thoughts. The use of benzodiazepines should be limited as they increase the risk of developing posttraumatic stress disorder. Vortioxetine becomes a very promising option. The most significant benefits of vortioxetine are the significant positive effects of vortioxetine on attention, memory, and executive function. There is some evidence for the use of alpha-1 adrenoceptor antagonists and alpha-2 adrenoceptor agonists in therapy. In insomnia the use of prazosin and trazodone is recommended. Pharmacotherapy of posttraumatic stress disorder requires administration of medications with multimodal action. Currently, there are no unified approaches to the pharmacotherapy of posttraumatic stress disorder. Further randomized clinical trials are necessary for developing effective treatment of posttraumatic stress disorder.

2023-01-01T00:00:00Z Appelhans, O. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/15466 2024-06-07T06:41:46Z 2023-01-01T00:00:00Z

Cómo ser una anatomista mujer en Ucrania Appelhans, O. La anatomía es una disciplina fundamental que es la base de todas las especialidades clínicas. El profesor debe poseer muchas habilidades quirúrgicas para enseñar a los estudiantes. El objetivo del estudio es analizar la igualdad de género en la educación médica en Ucrania. Todos los datos se obtuvieron de fuentes abiertas. Resultados. Las mujeres tienen potencial para obtener educación médica y enseñar a los estudiantes en las universidades médicas, también para realizar trabajos de investigación. Muchas mujeres dirigen los departamentos de Anatomía/Anatomía Clínica en Ucrania, pero están subrepresentadas entre los Rectores, Académicos y Ministros universitarios. Conclusiones. Sin embargo, las mujeres pueden trabajar duro, pueden realizar trabajos creativos, técnicos y organizativos con la misma calidad, aún tienen oportunidades limitadas para obtener los puestos administrativos más altos.

2023-01-01T00:00:00Z Koshelnyk, O. L. Vasilyev, V. V. Savenko, T. O. Кошельник, О. Л. Васильєв, В. В. Савенко, Т. О. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/15170 2024-04-30T11:40:50Z 2022-01-01T00:00:00Z

Changes in the endometrium with HIV-infection Koshelnyk, O. L.; Vasilyev, V. V.; Savenko, T. O.; Кошельник, О. Л.; Васильєв, В. В.; Савенко, Т. О. The purpose of our study was to evaluate the features of endometrial restructuring when infected with the human immunodeficiency virus. Many different studies have investigated the immunological and hormonal aspects.

2022-01-01T00:00:00Z Levchenko, O. V. Neskoromna, N. V. Prus, R. Chebotarova, S. O. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/15007 2024-04-05T21:17:36Z 2024-01-01T00:00:00Z

Rickets line: theory of an ideal profile Levchenko, O. V.; Neskoromna, N. V.; Prus, R.; Chebotarova, S. O. In the global medical context, aesthetic cosmetology has achieved the status of one of the most dynamically developed areas of contemporary global medicine. It successfully incorporates advanced technologies of medical practice, international trends in plastic surgery, scientific advances and so-called “soft” procedures to meet the individual needs of the skin patient ienta. The use of classic and new methods of anthropometry becomes crucial in the process of preparation before contour correction of the face. Analysis of individual characteristics of soft tissues using additional lateral cephalograms, which includes tracing the facial contour of the profile, is an invisible part of the strategy for successful aesthetic treatments. The use of such a technique is relevant not only in dental practice, but also in further aesthetic cosmetology with the method of eliminating unnecessary changes in the contour of soft tissues in order to improve the patient’s external appearance.

2024-01-01T00:00:00Z Levchenko, O. Sulima, V. Neskoromna, N. Chebotarova, S. https://repo.odmu.edu.ua:443/xmlui/handle/123456789/14900 2024-03-11T10:56:37Z 2024-01-01T00:00:00Z

The role of human face profile measurements for determining the need for correction in aesthetic cosmetology and orthodontics Levchenko, O.; Sulima, V.; Neskoromna, N.; Chebotarova, S. Aesthetic cosmetology in the global medical space is one of the constantly and dynamically progressing branches of medicine, which successfully combines the latest technologies, progressive global trends, scientific and practical achievements and noninvasive, so-called "soft" interventions under the conditions of an individual approach to each patient. Therefore, for the time being, it is extremely important to use modern methods of anthropometric measurements for further contour correction of various areas of the face. Analysis of soft tissues is carried out with the help of a lateral cephalogram, that is, the contour of the face is analyzed in profile, which later becomes an integral part of planning a successful aesthetic intervention. The use of this technique is advisable both in orthopedic dentistry and in aesthetic cosmetology for further forecasting changes in the contours of the soft tissues of the face in order to improve the patient's appearance.

2024-01-01T00:00:00Z